Gulcan Yuksekal
Sakarya University,Turkey
Title: Investigation of the relationship between chronic Spontaneous Urticaria and gut microbiome
Biography
Biography: Gulcan Yuksekal
Abstract
Intestinal microbiome is a large collection of bacteria, viruses, fungi and protozoa in our gastrointestinal (GIS) tract. Microbiome analyzes were also used to reveal the pathogenesis of some dermatological diseases such as acne vulgaris, psoriasis and atopic dermatitis. In addition to the skin microbiome, gut microbiome has a role in the pathogenesis of dermatological diseases.
In this study, we aimed to determine the possible relationship between CSU disease and gut microbiome, to determine the pathogenesis of CSU and to help new diagnosis and treatment approaches.
20 patients with CSU and 10 age and sex matched healthy individuals were included in the
present study. Both groups had not used a drug (antibiotics, proton pump inhibitors, etc.) or a probiotic/prebiotic that could affect gut microbiota for at least 4 weeks. After nucleic acid isolation from faecal samples, bacterial 16S ribosomal RNA (rRNA) gene target sequencing was performed with the Illumina MiSeq system using universal bacterial 16S primers (V3-V4). Statistical analyzes (LefSe, alpha and beta diversity) were performed after bioinformatics. The default settings (p <0.05 and LDA score> 2) identified taxa that differed significantly between the groups.
According to the results of series analysis; Bacteroidetes in patients with CSU and Actinobacteria phylum in healthy volunteers were significantly higher and Firmucites/Bacteroidetes ratio was lower in CSU patients.
As a result, there is a relationship between CSU disease and gut dysbiosis. Dysbiosis can be considered as an important factor in the pathogenesis of CSU and prebiotic or probiotics may be tried in the treatment.